Part:BBa_J18930:Design

mCerulean CFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• amino acid sequence taken from a pAG304GAL-Cerulean-ccdB from LindQuist lab

• borders trimmed to match the construct used for PDB 2Q57 (last 9 AA are not resolved in PDB)

• structure starts with MSK, this construct starts with MVSK but the M has been skipped for this Biobrick

• gene synthesis

• codon optimized for E. coli

Source

literature

gene synthesis